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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a vital function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two further splice variants were described but in depth nucleotide sequence information isn't nonetheless offered.

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Blocking peptides are peptides that bind specially towards the concentrate on antibody and block antibody binding. These peptide typically incorporates the epitope acknowledged because of the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This tutorial outlines further more feasible optimization of mobile and immunoassay parameters to ensure the best possible results are...

Antibodies sure to the blocking peptide not bind to the epitope within the goal protein. By evaluating the staining in the blocked antibody compared to the antibody alone, you can see which staining is unique.

Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and functions being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...

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